Journal: Frontiers in Synaptic Neuroscience
Article Title: Synaptic alterations and neuronal firing in human epileptic neocortical excitatory networks
doi: 10.3389/fnsyn.2023.1233569
Figure Lengend Snippet: Role of glutamate receptors in the generation of spontaneous synchronous population activity. (A) Recordings of spontaneous population activity (SPA) arising from different layers of a human neocortical slices, (B) SPAs and simultaneous single unit activities of a neocortical slice derived from a non-epileptic patient, during the consequent application of physiological solution – control, the NMDA receptor antagonist APV, APV + NBQX (NMDA and AMPA/kainate receptor antagonists), and finally physiological solution – washout. Local field potential gradients (LFPg), i.e., recordings filtered between 1 and 30 Hz are presented on the top traces, while lower extracts display the action potentials of single cells after high-pass filtering at 500 Hz. Asterisks indicate the occurrence of SPAs. Note the reduced presence of SPAs during APV bathing solution; while SPAs, but not all action potentials are abolished when NBQX is also applied. SPAs return after washing out of the pharmacological agents. Framed sections are displayed in detail on the right. Statistical comparisons between control conditions and application of APV for various parameters: (C) SPA recurrence frequency, (D) LFPg amplitudes and (E) multiunit activity (MUA) amplitudes. Note that the recurrence frequency of SPA has significantly decreased in epileptic tissue upon application of APV (* p < 0.05).
Article Snippet: Spontaneous population activity detection, LFPg and multiunit activity (MUA) were analyzed with the Neuroscan Edit4.5 program (Compumedics Neuroscan, Charlotte, NC, USA), as described in our previous study.
Techniques: Activity Assay, Derivative Assay, Control
